Personalia
- Leeftijd
- 40 jaar
Rijbewijs en talenkennis
Wat breng ik mee?
Zo omschrijven anderen mij
Excellent communication skills, especially in data presentation gained in international conferences and group presentations.
Team spirit gained through collaborations with other PhD students in other groups.Good ability to adapt to multicultural environments.
During my Master program I was elected for two times as student representative in the Faculty Board.
Good teaching skills, gained through teaching program during my PhD and by master and bachelor students supervision.
Extremely independent in work organization and planning, strict compliance to deadlines. Good organizational skills, applied both to planning, data and samples organization.
Experience with routine mantainace of lab equipment: during my PhD I was responsible for the routine manteinance of incubators and medium analyzer.
I was the responsible person for the ordering and storage of medium and cell culture supplements for the whole group. I was appointSelf-critical, analytical, organized.
Very autonomous in learning new techniques and proactive attitude.
Able to organize and follow different projects simultaneously.
My interdisciplinary formation and knowledge in the medical field enables me to interact with colleagues and collaborators from different fields of expertise and to connect researchers with medical doctors.
ed as chemistry laboratory supervisor and responsible person for the introduction to the laboratory safety rules.
Mijn huidige situatie
Werkervaring
PhD student
Universiteit Twente, Enschede (The Netherlands)
Strategies for the fabrication of an alginate-based three dimensional constructs for islet of Langerhans encapsulation and revascularization.
▪ Cell culture: beta cell lines (INS1E and MIN6), Islet of Langerhans, HUVEC, hMSC, human fibroblasts.
▪ Aggregation of cells for pseudo-islets production (INS1E, MIN6)
▪ Cell encapsulation in hydrogels (alginate, PEGDA)
▪ Broad experience in Glucose Induced Insulin Secretion Tests and ELISA assays
▪ Flow cytometry
▪ Evaluation of cell viability and metabolic activity
▪ UV-Vis spectrophotometry for protein and DNA content evaluation
▪ Fluorescent microscopy, fluorescent labeling
▪ Automated imaging using BD-Pathway
▪ Sprouting assays and tube formation assay, Image J quantification
▪ Evaluation of vascularization (chicken chorioallantoic membrane assay)
▪ 3D fiber deposition of thermoplastic polymers and plotting of hydrogels with 3D bio-plotters
▪ Functionalization of thermoplastic polymers (polycaprolactone) and polysaccharides (dextran, heparin).
▪ Bacteria culture and plasmid DNA isolation. Basics of eukariotic cell transfection and virus-mediated cell transduction.
Master thesis internship
University of Trieste, Department of Life Sciences, Trieste (Italy)
Development of an alginate-based filler for bone defects.
▪ Production of alginate microbeads with electronic droplet generator.
▪ Cell culture (MG63 and MC3T3)
▪ Cell proliferation, adhesion and cytotoxicity test
▪ Analysis of alginate gel structure and mechanical properties by rheology and NMR relaxometry
▪ Synthesis of peptides, capillary electrophoresis and mass spectroscopy
▪ Functionalization of polysaccharides with peptides for the improvement of materials bioactivity
▪ Microbiological tests for the evaluation of antibacterial activity
Bachelor thesis internship.
University of Trieste and SPRIN technologies S.P.A, Trieste (Italy)
www.sprintechnologies.com
Production and characterization of immobilized catalysts.
▪ Immobilization of enzymes on polymeric beads.
▪ Enzymatic activity tests.
▪ UV-vis spectroscopy for protein content determination.
▪ Titration and HPLC analysis and purification.
Opleidingen
Molecular biology, molecular medicine, immunology, biopolymers, biomaterials and tissue engineering, biochemistry, pharmacology and pathology. Proteomics and mass spectroscopy, microfabrication, microscopy. Knowledge of techniques such as AFM, RAMAN, FRAP, NMR, XPS.
Meer over mij
Zo besteed ik mijn vrije tijd
Ballet, modern dance, caraibic dance.
Reading
Werkgebied